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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: Hesperidin alleviates insulin resistance by improving HG-induced oxidative stress and mitochondrial dysfunction by restoring miR-149

Fig. 5

Knockdown of miR-149 partly reversed the biological effects of hesperidin. a The transfection efficiency of the miR-149 inhibitor was detected by qRT-PCR. b The effect of miR-149 downregulation on hesperidin-mediated ROS production was detected by a fluorescent MitoSox probe. c The effect of miR-149 downregulation on the hesperidin-regulated MMP level was determined by JC-1 staining. d, e The effects of miR-149 downregulation on hesperidin-regulated ATP and glucose contents were quantified by corresponding detection kits. f The effects of miR-149 downregulation on hesperidin-mediated phosphorylation of GSK3β, AKT and IRS1 were quantified by western blotting. All the data were presented as the mean ± S.D. *p < 0.05, **p < 0.01, *** p < 0.001

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