Molecular mechanisms of vitamin D plus Bisphenol A effects on adipogenesis in human adipose-derived mesenchymal stem cells

Table 1 Comparison of protein expression in vitamin D plus BPA groups vs. control

Proteins	Time	Group	Meana	Standard deviation	Result
FABP4	Day 6	Control	0.27	0.03	X = 6.5
		10 nM VitD + BPA	0.19	0.04	df = 2
		0.1 nM VitD + BPA	0.04	0.008	P-value = 0.038*
	Day 14	Control	0.32	0.02	X = 9. 04
		10 nM VitD + BPA	0.32	0.05	df = 2
		0.1 nM VitD + BPA	0.04	0.008	P-value = 0.01*
GLUT4	Day 6	Control	0.17	0.03	X = 9.75
		10 nM VitD + BPA	0.12	0.02	df = 2
		0.1 nM VitD + BPA	0.26	0.02	P-value = 0.007*
	Day 14	Control	0.20	0.01	X = 7. 80
		10 nM VitD + BPA	0.20	0.03	df = 2
		0.1 nM VitD + BPA	0.29	0.04	P-value = 0.02*
VDR	Day 6	Control	0.69	0.45	X = 5.02
		10 nM VitD + BPA	0.49	0.05	df = 2
		0.1 nM VitD + BPA	1.06	0.08	P-value = 0.08
	Day 14	Control	0.80	0.05	X = 8. 05
		10 nM VitD + BPA	0.82	0.05	df = 2
		0.1 nM VitD + BPA	1.12	0.17	P-value = 0.017*
ERβ	Day 6	Control	0.69	0.45	X = 5.02
		10 nM VitD + BPA	0.79	0.18	df = 2
		0.1 nM VitD + BPA	1.70	0.32	P-value = 0.08
	Day 14	Control	0.80	0.05	X = 8. 05
		10 nM VitD + BPA	1.32	0.71	df = 2
		0.1 nM VitD + BPA	2.00	0.56	P-value = 0.017*

Expression levels of adipogenic markers [GLUT4 protein and FABP4 protein] were increased in vitamin D plus BPA (10 nM) group, compared to control and concentration of 0.1 nM vitamin D plus BPA. In addition, protein expression of VDR and ERβ in both concentrations of vitamin D plus BPA vs. control was significantly increased
FABP4 fatty acid binding proteins-4, GLUT4 glucose transporter-4, VDR vitamin D receptor, ERβ estrogen receptor beta
*Kruskal–Wallis test was used and Mean values were significantly different between the groups (P < 0.05)
^ang/mg total protein

ISSN: 1758-5996