Fig. 6

Overexpression of miR-93-5p alleviated LPS-induced cell injury in HK2 cells by targeting OXSR1. a The protein level of OXSR1 in HK2 cells transfected with pcDNA or OXSR1 was measured by western blot. b–h LPS-treated HK2 cells were transfected with miR-NC, miR-93-5p, miR-93-5p+pcDNA or miR-93-5p+OXSR1. b The protein level of OXSR1 in LPS-induced HK2 cells after transfection was tested by western blot. c Cell viability in these cells was assessed by CCK-8 assay. d, e Cell apoptosis and percentage of cells at different stages were monitored by flow cytometry assay. f The concentration of IL-1β and TNF-α was examined by ELISA using corresponding kits. g, h The levels of SOD and MDA in these cells were examined by corresponding specific kits. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001