Fig. 3

Circ-FANCA directly interacted with miR-93-5p. a The complementary sequence between circ-FANCA and miR-93-5p were exhibited. b The overexpression efficiency of miR-93-5p in HK2 cells was evaluated by qRT-PCR. c, d The targeted relationship between circ-FANCA and miR-93-5p was validated by dual-luciferase reporter assay and RIP assay. e The expression level of miR-93-5p in the serums of septic AKI patients (n = 19) and healthy controls (n = 19) was examined by qRT-PCR assay. f Spearman’s correlation coefficient analysis was performed to estimate the correlation between circ-FANCA and miR-93-5p in the serums of septic AKI patients. g The level of miR-93-5p in LPS-stimulated HK2 cells was determined by qRT-PCR assay. h The expression level of circ-FANCA in LPS-induced HK2 cells transfected with circ-FANCA or pCD5-ciR was determined by qRT-PCR assay. i MiR-93-5p level was determined through qRT-PCR in LPS-induced HK2 cells following the transfection of si-NC, si-circ-FANCA, pCD5-ciR or circ-FANCA. **P < 0.01, ***P < 0.001, ****P < 0.0001