Skip to main content
Fig. 6 | Diabetology & Metabolic Syndrome

Fig. 6

From: Long non-coding RNA CDKN2B-AS1 regulates high glucose-induced human mesangial cell injury via regulating the miR-15b-5p/WNT2B axis

Fig. 6

MiR-15b-5p regulated DN development through WNT2B. a–f HMCs were transfected with miR-NC, miR-15b-5p, miR-15b-5p + pcDNA, or miR-15b-5p + WNT2B. a The level of WNT2B protein in HMCs after HG treatment was detected with western blotting, and GADPH was selected as an internal control. b, c The viability and cell cycle progression of HMCs under HG treatment were determined via CCK-8 or flow cytometry assays. d, e The levels of PCNA, CyclinD1, Fibronectin, and Collagen IV in HMCs under HG treatment were evaluated by western blotting, and GADPH was selected as an internal control. f The levels of IL-6, IL-1β, and TNF-α in HMCs after HG treatment were analyzed by ELISA. The experiments were repeated 3 times, and data were presented as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001

Back to article page