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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Long non-coding RNA CDKN2B-AS1 regulates high glucose-induced human mesangial cell injury via regulating the miR-15b-5p/WNT2B axis

Fig. 4

CDKN2B-AS1 regulated DN progression by sponging miR-15b-5p in HMCs. a–f HMCs were transfected with si-NC, si-CDKN2B-AS1, si-CDKN2B-AS1 + anti-miR-NC, or si-CDKN2B-AS1 + anti-miR-15b-5p. a Expression of miR-15b-5p in HG-treated HMCs was detected by qRT-PCR, and U6 was selected as an internal reference. b, c CCK-8 and flow cytometry assays were employed to analyze the proliferation or cell cycle progression of HG-treated HMCs. d, e Western blotting was executed to assess the levels of PCNA, CyclinD1, Fibronectin, and Collagen IV in HG-treated HMCs, and GADPH was selected as an internal control. f ELISA was conducted to evaluate the levels of IL-6, IL-1β, and TNF-α in HG-induced HMCs. The experiments were repeated 3 times, and data were presented as mean ± standard deviation. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001

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