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Fig. 5 | Diabetology & Metabolic Syndrome

Fig. 5

From: Compromised browning plasticity of primary subcutaneous adipocytes derived from overweight Chinese adults

Fig. 5

Oxygen consumption rate in differentiated adipocytes. The differentiated adipocytes derived from the normal weight (NW) and overweight (OW) groups treated with (NW-NE and OW-NE) or without (NW-con and OW-con) norepinephrine were used to detect oxygen consumption rate (OCR). The oxygen consumption rate (OCR) was measured at baseline and followed by sequential stimulation with oligomycin, FCCP, and antimycin A. Subtracting antimycin A-insensitive OCR (non-mitochondrial respiration) from baseline OCR reveals basal OCR, oligomycin was added to render uncoupled OCR, and FCCP rates represent maximal respiratory capacity, three data points were collected from each period, and mean value of the three points were calculated for data analysis. Experiments were repeated at least 3 times. a Basal, uncoupled and maximal OCR values were normalized by the total-cell protein concentration and compared between different groups (b). (Values were mean ± SD; n = 10 for the NW-NE, NW-con, OW-NE and OW-con groups; data used for analysis were mean values of four replicate wells for each sample. #P < 0.05 NW vs OW group; *P < 0.05 control vs NE treatment). NW-NE normal weight norepinephrine treatment group, NW-con normal weight control group, OW-NE overweight norepinephrine treatment group, OW-con overweight control group, OCR oxygen consumption rate

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