Fig. 5

KCNQ1OT1 targeted miR-18b-5p to regulate SORBS2 expression. a the putative binding sites between KCNQ1OT1 and miR-18b-5p were predicated by starBase 3.0. b The luciferase activities of WT-KCNQ1OT1 and MUT-KCNQ1OT1 were measured in 293 T cells transfected with miR-NC or miR-18b-5p. c The expression of miR-18b-5p was detected by qRT-PCR in HGMCs and HRGECs transfected with pcDNA-KCNQ1OT1. d The expression of miR-18b-5p was detected by qRT-PCR in HGMCs and HRGECs transfected with miR-18b-5p inhibitor. e, f proliferation of HGMCs and HRGECs was determined after transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + miR-18b-5p inhibitor. g, h apoptosis of HGMCs and HRGECs was determined after transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + miR-18b-5p inhibitor. i the fibrosis-related proteins were detected by western blot after transfection with si-KCNQ1OT1 or si-KCNQ1OT1 + miR-18b-5p inhibitor. j The putative binding sites between miR-18b-5p and SORBS2 were predicated by starBase 3.0. k The luciferase activities of WT-SORBS2 and MUT- SORBS2 were measured in 293 T cells transfected with miR-NC or miR-18b-5p. l, m, the mRNA and protein levels of SORBS2 were detected in HGMCs and HRGECs transfected with miR-18b-5p or miR-18b-5p + pcDNA-KCNQ1OT1. N = 3, *P < 0.05