Fig. 4

KCNQ1OT1 affected proliferation and apoptosis by regulating SORBS2 expression in high glucose-induced HGMCs and HRGECs. a, b the mRNA level of SORBS2 was detected by qRT-PCR in HGMCs and HRGECs transfected with si-KCNQ1OT1 or pcDNA-KCNQ1OT1. c, d the protein level of SORBS2 was detected by western blot in HGMCs and HRGECs transfected with si-KCNQ1OT1 or pcDNA-KCNQ1OT1. e the protein level of SORBS2 was detected in HGMCs and HRGECs transfected with pcDNA-SORBS2. f, g, proliferation of HGMCs and HRGECs was determined after transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-SORBS2. h and i apoptosis of HGMCs and HRGECs was determined after transfected with si-KCNQ1OT1 or si-KCNQ1OT1 + pcDNA-SORBS2. j western blot assay was utilized to detect the fibrosis-related proteins. N = 3, *P < 0.05