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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Long non-coding RNA cancer susceptibility candidate 2 (CASC2) alleviates the high glucose-induced injury of CIHP-1 cells via regulating miR-9-5p/PPARγ axis in diabetes nephropathy

Fig. 4

CASC2 acted as a ceRNA by sponging miR-9-5p to facilitate PPARγ expression. a The mRNA expression of PPARγ in NG, HG or mannitol-treated CIHP-1 cells was analyzed by qRT-PCR. b qRT-PCR assay was used to measure the mRNA expression of PPARγ in CIHP-1 cells treated by HG (30 mM) at different times. c The protein expression of PPARγ in NG, HG or mannitol-treated CIHP-1 cells was analyzed by western blot assay. d Western blot assay was used to measure the protein expression of PPARγ in CIHP-1 cells treated by HG (30 mM) at different times. e StarBase v2.0 predicted that there were binding sites between miR-9-5p and PPARγ. f Dual luciferase reporter assay was conducted to detect the interaction between miR-9-5p and PPARγ in CIHP-1 cells. g The expression of miR-9-5p in CIHP-1 cells transfected with anti-NC or anti-miR-9-5p was measured by qRT-PCR. h The protein expression of PPARγ in CIHP-1 cells transfected with miR-NC, miR-9-5p, anti-NC or anti-miR-9-5p was assessed using western blot assay. i PPARγ protein expression in CIHP-1 cells transfected with si-NC, si-CASC2, si-CASC2 + anti-NC, or si-CASC2 + anti-miR-9-5p was estimated by western blot. *P < 0.05

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