Skip to main content
Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Long non-coding RNA cancer susceptibility candidate 2 (CASC2) alleviates the high glucose-induced injury of CIHP-1 cells via regulating miR-9-5p/PPARγ axis in diabetes nephropathy

Fig. 1

CASC2 alleviated the HG-induced podocytes injury. a The expression of CASC2in CIHP-1 cells treated with normal glucose (NG), high glucose (HG) or mannitol was detected by qRT-PCR. b After CIHP-1 cells were treated with HG (mM) for 12 h, 24 h and 48 h, respectively, CASC2 expression was measured by qRT-PCR. c CIHP-1 cells were divided into four groups, which were control, NG (5 mM),  HG (30 mM), HG  + vector and HG + CASC2, CASC2 expression was detected by qRT-PCR. d Cell viability was assessed by CCK-8 assay. e Cell apoptosis was examined by flow cytometry. f, g Western blot assay was used to determine the expression levels of apoptosis-related proteins BCL-2 and Cleaved-caspase-3 and autophagy related proteins LC3-II, LC3-I and Beclin 1. *P < 0.05

Back to article page