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Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Impact of intracellular toxic advanced glycation end-products (TAGE) on murine myoblast cell death

Fig. 1

Cell viability and intracellular TAGE levels in C2C12 cells treated with glyceraldehyde and aminoguanidine. GA: glyceraldehyde. AG: aminoguanidine. a, b Cells were treated with 0, 0.5, 1, 1.5, and 2 mM GA for 24 h. c, d Cells were pretreated with 0 or 8 mM AG for 2 h, followed by 0, 1.5, and 2 mM GA for 24 h. a, c Cell viability was assessed by the WST-8 assay, which was performed in three independent experiments. One experiment was performed using 7 wells to calculate the average. Data are shown as mean ± S.D. (N = 3). b, d Intracellular TAGE were analyzed using a slot blot analysis. Cell lysates (2.0 μg of protein/lane) were blotted onto a polyvinylidene difluoride membrane. The densities of HRP-linked molecular marker bands were used to correct for differences in densities between membranes. The amount of TAGE was calculated based on a calibration curve for TAGE-BSA. A slot blot analysis was performed in three independent experiments. One experiment was performed using 2 lanes to calculate the average. Data are shown as mean ± S.D. (N = 3). a, b P-values were based on the Bonferroni test. *p < 0.05 vs. 0 mM GA. **p < 0.01 vs. 0 mM GA. #p < 0.05 vs. 1.5 mM GA. c, d P-values were based on Tukey’s test. **p < 0.01 vs. 0 mM GA without AG. #p < 0.05 vs. 1.5 mM GA without AG. ##p < 0.01 vs. 1.5 mM GA without AG. ++p < 0.01 vs 2 mM GA without AG

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