Fig. 2

The different values of exosome were used for the determination of the dose. a The different doses of 0.25–64 µg/mL were applied in the MTT assay that the doses of 0.25–4 µg/mL were the optimal doses for the next experiments. b The phosphorylation of GSK3β at Ser-9 position was determined in the cells treated with the doses of 2 and 4 µg/mL PN-Exo and PO-Exo using western blot analysis that c based on p-GSK3β/GSK3β ratio, the dose of 4 µg/mL were detected as the optimal dose for the future treatments (P = 0.014). PN-Exo; pooled exosomes obtained from normal-weight women, PO-Exo; pooled exosomes obtained from obese women. The data are presented as median ± IQR