Table 1 Primer sequences for real-time PCR
From: Long-term treatment with metformin in the prevention of fatty liver in Zucker diabetic fatty rats
Gene name | Primer sequences | |
|---|---|---|
G6pdx | Forward | 5′-GAC TGT GGG CAA GCT CCT CAA-3′ |
Reverse | 5′-GCT AGT GTG GCT ATG GGC AGG T-3′ | |
Hmgcs1 | Forward | 5′-TCA AGG CTT GAC TCA AGA ACG-3′ |
Reverse | 5′-GGA ATA TGC TCT GTA GCT GTG-3′ | |
Cyp7a1 | Forward | 5′-ATG ACA CGC TCT CCA CCT TTG A-3′ |
Reverse | 5′-AGC TCT TGG CCA GCA CTC TGT-3′ | |
Igfbp1 | Forward | 5′-GAA GCT TTT CTC ATC TCC ATA CAT GT-3′ |
Reverse | 5′-AAG GCC CCT ACC TCA GAC TGA-3′ | |
Scd-1 | Forward | 5′-GCT TGT GGA GCC ACA GGA CT-3′ |
Reverse | 5′-ATC CCG GGC CCA TTC ATA-3′ | |