Fig. 4
From: Long-term treatment with metformin in the prevention of fatty liver in Zucker diabetic fatty rats
Real-time PCR quantification of target genes. Total mRNA of liver tissues were extracted from Zucker lean rats (ZL), vehicle-treated ZDF rats (V) and metformin-treated ZDF rats (Met) after 6-month treatment. Genes with interests were validated by quantitative real-time PCR. Similar to the results of microarray assay, gene expression levels of G6PDX, HMGCS1, CYP7a1 and IGFBP1were modified by leptin receptor mutation and corrected by metformin treatment, while the expression level of SCD-1 was not corrected by metformin treatment. Data are mean ± SD, *p < 0.05, V versus ZL; #p < 0.05, Met versus V