Fig. 1
From: Effects of metformin on metabolism of white and brown adipose tissue in obese C57BL/6J mice
Work flow of the present study. C57BL/6J mice were fed with normal diet (ND) or high-fat diet (HFD) for 22 weeks. Six of HFD-induced obese mice were treated with metformin. After treatment for 8 weeks, oral glucose tolerance test (OGTT) and hyperinsulinemic–euglycemic clamp were performed to evaluate the improvement of glucose tolerance and insulin resistance. Protein expressions of WAT and BAT in mice among ND, HFD, and MET group were identified and quantified with iTRAQ-coupled 2D LC-MS/MS. The results were analyzed by MASCOT, Scaffold and IPA. Finally, western blot analysis was performed to validate the variation of the key differential proteins