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Fig. 4 | Diabetology & Metabolic Syndrome

Fig. 4

From: Possible involvement of normalized Pin1 expression level and AMPK activation in the molecular mechanisms underlying renal protective effects of SGLT2 inhibitors in mice

Fig. 4

Canagliflozin activates AMPK and inhibits the proliferation of mesangial CRL1927 cells. a C57BL/6J mice were administered canagliflozin by intravenous injection. Two hours later, the kidneys were extirpated and lysates were immunoblotted using actin, anti-phosphorylated AMPK and anti-AMPK antibodies. (n = 4). b CRL1927 cells were treated without or with 2 μM, 5 μM and 10 μM canagliflozin for 1 h. The cell lysates were immunoblotted using anti-acetyl CoA carboxylase (pACC), anti-ACC, anti-phosphorylated AMPK and anti-AMPK antibodies. Representative data from three independent experiments are shown. c CRL1927 cells were exposed to canagliflozin at the indicated concentration for 1 h. Then, an ADP/ATP ratio assay was performed. (n = 5). d Cell proliferation assessed by MTT assay in CRL1927 cells cultured with 0 μM, 2 μM, 5 μM and 10 μM canagliflozin for 24 h. (n = 6). e Cell proliferation assessed by MTT assay in CRL1927 cells cultured with or without 5 μM compound C and 10 μM canagliflozin for 24 h. (n = 6). f Cell proliferation assessed by MTT assay in CRL1927 cells cultured with 5 mM 2DG and 0.5 mM AICAR for 24 h. (n = 6). g CRL1927 cells were exposed to 100 μM canagliflozin or hydroxyperoxide for 24 h. The cells were stained with 10 μg/ml propidium iodide for 1 h and then observed. h Canagliflozin was applied to CRL1927 cells for 24 h. Then, protein levels were examined. Representative data from two-independent experiments are shown. *P < 0.05, **P < 0.01

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