Fig. 4From: Possible involvement of normalized Pin1 expression level and AMPK activation in the molecular mechanisms underlying renal protective effects of SGLT2 inhibitors in miceCanagliflozin activates AMPK and inhibits the proliferation of mesangial CRL1927 cells. a C57BL/6J mice were administered canagliflozin by intravenous injection. Two hours later, the kidneys were extirpated and lysates were immunoblotted using actin, anti-phosphorylated AMPK and anti-AMPK antibodies. (n = 4). b CRL1927 cells were treated without or with 2 μM, 5 μM and 10 μM canagliflozin for 1 h. The cell lysates were immunoblotted using anti-acetyl CoA carboxylase (pACC), anti-ACC, anti-phosphorylated AMPK and anti-AMPK antibodies. Representative data from three independent experiments are shown. c CRL1927 cells were exposed to canagliflozin at the indicated concentration for 1 h. Then, an ADP/ATP ratio assay was performed. (n = 5). d Cell proliferation assessed by MTT assay in CRL1927 cells cultured with 0 μM, 2 μM, 5 μM and 10 μM canagliflozin for 24 h. (n = 6). e Cell proliferation assessed by MTT assay in CRL1927 cells cultured with or without 5 μM compound C and 10 μM canagliflozin for 24 h. (n = 6). f Cell proliferation assessed by MTT assay in CRL1927 cells cultured with 5 mM 2DG and 0.5 mM AICAR for 24 h. (n = 6). g CRL1927 cells were exposed to 100 μM canagliflozin or hydroxyperoxide for 24 h. The cells were stained with 10 μg/ml propidium iodide for 1 h and then observed. h Canagliflozin was applied to CRL1927 cells for 24 h. Then, protein levels were examined. Representative data from two-independent experiments are shown. *P < 0.05, **P < 0.01Back to article page