Fig. 3From: Possible involvement of normalized Pin1 expression level and AMPK activation in the molecular mechanisms underlying renal protective effects of SGLT2 inhibitors in miceRenal Pin1 expression was markedly increased in the NA/STZ/Suc mice and was normalized by canagliflozin treatment. a Whole kidneys were extirpated from each mouse and Pin1 expression levels were examined by immunoblotting. (n = 5). b Relative mRNA levels of Pin1 in mouse kidneys. (n = 6). c Immunostaining of the renal sections with anti-Pin1 antibody. d Double immunostaining with anti-Pin1 (brown staining) and anti-PDGFRβ (blue staining as a mesangial marker). Scale bars: 50 μm. e Cell proliferation assessed by MTT assay in CRL1927 Pin1 KD cells. (n = 6). *,#P < 0.05, **P < 0.01, ***P < 0.001Back to article page