Fig. 3

Renal Pin1 expression was markedly increased in the NA/STZ/Suc mice and was normalized by canagliflozin treatment. a Whole kidneys were extirpated from each mouse and Pin1 expression levels were examined by immunoblotting. (n = 5). b Relative mRNA levels of Pin1 in mouse kidneys. (n = 6). c Immunostaining of the renal sections with anti-Pin1 antibody. d Double immunostaining with anti-Pin1 (brown staining) and anti-PDGFRβ (blue staining as a mesangial marker). Scale bars: 50 μm. e Cell proliferation assessed by MTT assay in CRL1927 Pin1 KD cells. (n = 6). *^,#P < 0.05, **P < 0.01, ***P < 0.001