Table 2 The location of TCF7L2-binding motif in the promoter of PIK3R1 and primers used to amplify CAA-mutated fragments
From: TCF7L2 regulates pancreatic β-cell function through PI3K/AKT signal pathway
Location of TCF7L2-binding motif | Forward | Reverse |
|---|---|---|
381–384 | 5′-TTTTCCGTTAATGGTTAAGAAGTGGGGGCT-3′ | 5′-AGCCCCCACTTCTTAACCATTAACGGAAAA-3′ |
487–490 | 5′-TTAGCTGCAACTTTCTATTACAGACTAGAA-3′ | 5′-TTCTAGTCTGTAATAGAAAGTTGCAGCTAA-3′ |
1795–1798 | 5′-GCAGGAAGTTGGGGTGCCAGGGCTTGCAGG-3′ | 5′-CCTGCAAGCCCTGGCACCCCAACTTCCTGC-3′ |
1986–1989 | 5′-CTTTTGGGAGAAGACTCGTTTTAAATTAAA-3′ | 5′-TTTAATTTAAAACGAGTCTTCTCCCAAAAG-3′ |
2387–2390 | 5′-CCATCCATGTAAATGTGAGGTTCTGGCTCC-3′ | 5′-GGAGCCAGAACCTCACATTTACATGGATGG-3′ |
2420–2423 | 5′-CAGGGGCAACGTATATGCTCCAGTCTTGGT-3′ | 5′-ACCAAGACTGGAGCATATACGTTGCCCCTG-3′ |
3232–3235 | 5′-AGCTAGGCTGATTTTACTGAG-3′ | 5′-TTCAGCACAGGAATGAAGCTG-3′ |