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Table 2 The location of TCF7L2-binding motif in the promoter of PIK3R1 and primers used to amplify CAA-mutated fragments

From: TCF7L2 regulates pancreatic β-cell function through PI3K/AKT signal pathway

Location of TCF7L2-binding motif Forward Reverse
381–384 5′-TTTTCCGTTAATGGTTAAGAAGTGGGGGCT-3′ 5′-AGCCCCCACTTCTTAACCATTAACGGAAAA-3′
487–490 5′-TTAGCTGCAACTTTCTATTACAGACTAGAA-3′ 5′-TTCTAGTCTGTAATAGAAAGTTGCAGCTAA-3′
1795–1798 5′-GCAGGAAGTTGGGGTGCCAGGGCTTGCAGG-3′ 5′-CCTGCAAGCCCTGGCACCCCAACTTCCTGC-3′
1986–1989 5′-CTTTTGGGAGAAGACTCGTTTTAAATTAAA-3′ 5′-TTTAATTTAAAACGAGTCTTCTCCCAAAAG-3′
2387–2390 5′-CCATCCATGTAAATGTGAGGTTCTGGCTCC-3′ 5′-GGAGCCAGAACCTCACATTTACATGGATGG-3′
2420–2423 5′-CAGGGGCAACGTATATGCTCCAGTCTTGGT-3′ 5′-ACCAAGACTGGAGCATATACGTTGCCCCTG-3′
3232–3235 5′-AGCTAGGCTGATTTTACTGAG-3′ 5′-TTCAGCACAGGAATGAAGCTG-3′