Table 1 PCR primers used to amplify contiguous fragments of PIK3R1 promoter
From: TCF7L2 regulates pancreatic β-cell function through PI3K/AKT signal pathway
Primer | Forward | Reverse |
|---|---|---|
1 | 5′-TTGTTCTGAGACAAAATAGAC-3′ | 5′-CCACTTCTTTTGAACCATTAA-3′ |
2 | 5′-TTAATGGTTCAAAAGAAGTGG-3′ | 5′-CAGTCAGCCCCGCAGGTGTTA-3′ |
3 | 5′-TAACACCTGCGGGGCTGACTG-3′ | 5′-GGGCAGGTTCAAATATTTGCT-3′ |
4 | 5′-AGCAAATATTTGAACCTGCCC-3′ | 5′-ACTTTGCAGGTTATGCATTTC-3′ |
5 | 5′-GAAATGCATAACCTGCAAAGT-3′ | 5′-CTCAGTAAAATCAGCCTAGCT-3′ |
6 | 5′-AGCTAGGCTGATTTTACTGAG-3′ | 5′-GGCTTTGTGGCTGTGAATGTT-3′ |
7 | 5′-AACATTCACAGCCACAAAGCC-3′ | 5′-TGCTCAATAGTGGTGAGTTTC-3′ |
8 | 5′-GAAACTCACCACTATTGAGCA-3′ | 5′-CGTGCATCTGGGTTTAGAGAT-3′ |
9 | 5′-ATCTCTAAACCCAGATGCACG-3′ | 5′-AGTCCGGCTTTCTTTGTAATG-3′ |
10 | 5′-TTATCAGCTCTCGTCAATCTGC-3′ | 5′-TGTGCGACAGTTTCCTTGGCT-3′ |