Fig. 1

Isolation and biological characterization of SHED. Flow cytometry analysis revealed that SHED express the MSC markers CD73 (a), CD90 (b) and CD105 (c), while lack expression in blood cell marker CD45 (d). SHED have the ability of multilineages of differentiation. They could be induced to form mineralized nodules, lipid droplets and chondrocytes, as indicated by arrows in (e–g), respectively. Developmental potential of SHED ex vivo