Fig. 6
Modifying effects of IL-6, IL-1β and 1α,25(OH)2D3 on the phosphorylation of relA and p44/42 MAPK in MacCM-stimulated human white preadipocytes. Preadipocytes were either cultured alone (control), or with THP-1-MacCM (25%), or in the presence of IL-6 antibody (300 ng/ml), or IL-1β antibody (15 μg/ml) for 24 h. A further group of cells was pre-treated with 1α,25(OH)2D3 (10 nM) for 24 h, followed by treatments with THP-1-MacCM (25%) and 1α,25(OH)2D3 (10 nM) for a further 24 h before lysate collection. a The levels of relA, phosphorylated relA, p44/42 MAPK and phosphorylated p44/42 MAPK were measured by western blotting. The results are presented as fold changes of ratios of b phosphorylated relA to relA, c phosphorylated p44/42 MAPK to p44/42 MAPK to controls. Data are shown as mean ± SEM for groups of 6. The results were analyzed using one-way ANOVA with Tukey’s post hoc test and confirmed by three independent experiments. A significant difference to control was indicated by ***(p < 0.001); to THP-1-MacCM by #(p < 0.05), ##(p < 0.01) and ###(p < 0.001)