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Fig. 3 | Diabetology & Metabolic Syndrome

Fig. 3

From: 1α,25(OH)2D3 attenuates IL-6 and IL-1β-mediated inflammatory responses in macrophage conditioned medium-stimulated human white preadipocytes by modulating p44/42 MAPK and NF-κB signaling pathways

Fig. 3

Effects of 1α,25(OH)2D3 on IL-6 and IL-1β-mediated pro-inflammatory gene expression in MacCM-stimulated human white preadipocytes. Preadipocytes were either cultured alone (control), or with THP-1-MacCM (25%), or in the presence of IL-6 antibody (300 ng/ml), or IL-1β antibody (15 μg/ml) for 24 h. A further group of cells was pre-treated with 1α,25(OH)2D3 (10 nM) for 24 h, followed by treatments with THP-1-MacCM (25%) and 1α,25(OH)2D3 (10 nM) for a further 24 h before cell collection. The mRNA levels of pro-inflammatory factors a IL-1β, b IL-6, c IL-8, d MCP-1 and e RANTES were measured by qPCR. Data are shown as mean ± SEM for groups of 6. The results were analyzed using one-way ANOVA with Tukey’s post hoc test and confirmed by three independent experiments. A significant difference to control was indicated by ***(p < 0.001); to THP-1-MacCM by #(p < 0.05), ##(p < 0.01) and ###(p < 0.001)

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