Fig. 2

Retinal cross section. Immunofluorescent analyses of diabetic and control animals without purinergic receptors antagonist treatment. A Diabetic group (34 weeks after STZ IP injection). B Control group. P2Y2r immunoreactivity was seen in the fiber layer of diabetic and control animals (arrow-A1 and B1). In the diabetic group staining had a larger extension. Positive immunoreactivity of GFAP was observed in glial cells of diabetics and controls (arrow A2 and B2). In diabetic animals immunoreactivity was much more extended and some GFAP positive cells showed a morphology similar to that seen in Müller Cells (arrow head-A2). Co-expression of P2Y2 and GFAP in diabetics and controls had different patterns. In diabetic rats co-expression of P2Y2 and GFAP was seen in the fiber layer and in glial cells extending to the photoreceptor layer (A3), while in controls the co-expression was slightly observed in the fiber layer (B3)