Table 1 Primers used for the analysis of adiponectin and resistin expression on mRNA level in adipose tissues
From: Adiponectin/resistin interplay in serum and in adipose tissue of obese and normal-weight individuals
Gene | Gene description | Gene bank | Primers | Annealing (oC) | |
|---|---|---|---|---|---|
ADIPOQ | Adiponectin | NM_001177800.1 | F | 5′GGTCTCGAACTCCTGGCCTA3′ | 60 |
R | 5′TGAGATATCGACTGGGCATGGT3′ | ||||
RETN | Resisitin | NM_020415.3 | F | 5′GCTGTTGGTGTCTAGCAAGAC3′ | 59 |
R | 5′CATCATCATCATCATCTCCAG3′ | ||||
ACTB | β-Actin | NM_001101 | F | 5′CAGCCTGGATAGCAACG-TACA3′ | 61 |
R | 5′TTCTACAATGAGCTGCGTGTG3′ | ||||