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Fig. 6 | Diabetology & Metabolic Syndrome

Fig. 6

From: The promotion of nephropathy by Porphyromonas gingivalis lipopolysaccharide via toll-like receptors

Fig. 6

Expressied amounts of TLR2, TGF-β, and type 1 collagen in the STZ-induced type 1 diabetic mouse glomeruli. Areas immunostained by anti-podoplanin, anti-TLR2, anti-TGF-β, and anti-type 1 collagen were measured around different glomeruli (10/section) in laser-scanned microscopic images at 819x magnification by ImageJ. The relative expressed amounts of anti-TLR2, anti-TGF-β, and anti-type 1 collagen were expressed by the mean of the ratio: the area of anti-TLR2, anti-TGF-β, and anti-type 1 collagen in a glomerulus/area of a glomerulus within the podoplanin-positive podocytes. Expression of TLR2 and TGF-β in glomeruli was almost fully absent in the non-diabetic ICR mice (ICR) and in the non-diabetic ICR mice administered Pg-LPS (LPS), but TLR2 and TGF-β was detected in the STZ-induced type 1 diabetic mice (STZ). Expressed amounts of TLR2 and TGF-β in glomeruli were much higher in the diabetic mice administered Pg-LPS (STZ-LPS) and the level was lower in the diabetic mice administered both Pg-LPS and eritoran (eritoran). Type 1 collagen was detected in the non-diabetic ICR mice (ICR) and non-diabetic ICR mice administered Pg-LPS (LPS), and the expressed amount was lower in the STZ-induced type 1 diabetic mice (STZ). The type 1 collagen accumulation in the glomeruli was much higher in the diabetic mice administered Pg-LPS (STZ-LPS) and the level was lower in the eritoran-administered diabetic mice with Pg-LPS (eritoran). Data are expressed as means + SD. *Significantly different in the one-way ANOVA (p < 0.01)

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