Fig. 3From: Serotonin improves glucose metabolism by Serotonylation of the small GTPase Rab4 in L6 skeletal muscle cells 5-HT increases GLUT-4 translocation. L6 cells were incubated with 10 µM 5-HT or mHT in the absence or presence of 100 nM insulin for 1 h. After cell lysis, the lysates were used for membrane protein isolation followed by immunoblotting (IB) with an antibody to GLUT4. Detection of pan-cadherin (p-Cad) was used as a loading control. Significant differences are indicated as **P < 0.01. (N = 4 in duplicate)Back to article page