Fig. 2

Intragastric administration of 3DG for 2 weeks led to a decrease in GLP-1 secretion in rats n = 6 for each group. After 2 weeks of intragastric administration of 3DG, an OGTT was performed in rats fasted overnight. Plasma total GLP-1 concentrations (a) were measured with ELISA kits before and after oral glucose load (2.5 g/kg) in 3DG (5, 20, 50 mg/kg) or vehicle treated rats. b Basal plasma DPP-4 activity was measured in 3DG (5, 20, 50 mg/kg) or vehicle treated rats. c STC-1 cells were exposed to different concentrations of 3DG (80, 300, 1000 ng/mL) for 6 h, and thereafter GLP-1 concentration in the supernatant was measured by ELISA. d STC-1 cells were treated with different concentrations of 3DG (80, 300, 1000 ng/mL) for 12 h and thereafter their viability was measured using an MTT assay. Values are mean ± SD. *p < 0.05, **p < 0.01 compared with control group at the same point (a). *p < 0.05, **p < 0.01, ***p < 0.001 compared with control group (a, b, c, d)