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Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: β-Cell death is decreased in women with gestational diabetes mellitus

Fig. 1

A schematic depiction of β-cell derived insulin DNA biomarker assay. DNA was extracted from 300 μL of serum and converted with bisulfite treatment. Converted DNA was subjected to a first step PCR amplification followed by gel electrophoresis. Gel purified amplicons were tested for the presence of β-cell derived insulin DNA by real-time PCR using methylation-sensitive probes. The relative abundance of demethylated DNA was expressed using the following equation: demethylation index = 2(methylated cycle number) − (demethylated cycle number)

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