Fig. 5

DPP-4 inhibitor increased β cell mass and ameliorated islet morphology in SL- or SO-fed db/db mice. The experiments were performed in 14-week-old mice, as shown in Fig. 2. a Representative pancreatic sections stained with antibodies for insulin (brown) are shown. The scale bar represents 100 μm. b β cell mass (n = 6–8). The β cell area is shown as a proportion of the area of the entire pancreas. c Representative pancreatic sections stained with antibodies for insulin (green) and glucagon (red) are shown. The scale bar represents 50 μm. d Quantification of β cell mass as a proportion of the total α-cell-plus-β-cell mass in the islet (n = 6). *P < 0.05 vs. db/db SL. ^†P < 0.05 vs. db/db SO