Fig. 4
From: Progesterone ameliorates diabetic nephropathy in streptozotocin-induced diabetic Rats
Immunohistochemistry and real-time PCR detect that progesterone replacement increase the MMP-2 protein, but not the mRNA levels in the diabetic kidney. A Immunohistochemical stain of the kidney sections (hematoxylin staining; magnification, ×400) show that the MMP-2 immunostaining (brown staining) in the glomeruli was much stronger in the ND group compared with the D and the D-OVX groups (arrows). Progesterone replacement inhibited the decrease in the MMP-2 immunostaining in the D + OVX + P group. B MMP-2 mRNA expressions by real-time PCR. Data represent the mean ± SEM. Means with different superscript letters are significantly different from one another (P < 0.05)