Fig. 5
From: High glucose induces and activates Toll-like receptor 4 in endothelial cells of diabetic retinopathy
Inhibition of TLR4 reduces high glucose-induced production of IL-1β by HMEC-1 cells. a Western blot analysis of TLR4 expression by HMEC-1 cells under high glucose condition after siRNA treatment. NG normal glucose, HG high glucose, Sc scrambled control siRNA, si2 TLR4 siRNA 0.2 pmol/l, si4 TLR4 siRNA 0.4 pmol/l, si8 TLR4 siRNA 0.8 pmol/l. b IL-1β concentration in supernatants of HMEC-1 cells after high-glucose treatment in the presence of TLR4 siRNA (0.8 pmol/l) was measured using ELISA. Asterisk indicates P < 0.05 compared to 5.5 mmol/l glucose. c IL-1β level in supernatants of HMEC-1 cells in the presence of TLR4 antagonist LPS-RS (1 μg/ml) after high-glucose treatment was measured using ELISA. Asterisk indicates P < 0.05 compared to scrambled control RNA