Fig. 3

Expression of TLR4 in HMEC-1 cells subjected to high glucose. a HMEC-1 cells were stimulated with glucose at the doses of 15 and 25 mmol/l for 6 h. The mRNA for TLR4 was detected by quantitative RT-PCR and normalized to GAPDH. Asterisk indicates P < 0.05 compared to 5.5 mmol/l glucose. b The cells were stimulated with 15 mmol/l glucose for 0, 1, 3, 6, 12 and 24 h. The mRNA of TLR4 was detected by quantitative RT-PCR and normalized to GAPDH and expressed as the mean ± SE. *P < 0.05 compared to the 0 h group. c HMEC-1 cells were treated with 5.5, 15 and 25 mmol/l glucose for 6, 12 and 24 h and western blot was performed. β-actin was used as a control. d TLR4 expression on HMEC-1 cells challenged with 15 mmol/l glucose for 24 h assessed by flow cytometry