Fig. 6

IRAK-1 gene expression in the adipose tissue correlates with TLR2 and/or MyD88 gene expression. The adipose tissue gene expression of IRAK-1 and upstream signaling components of the TLR/IL-1R/MyD88 pathway including TLR2, TLR4, and MyD88 was determined in 49 non-diabetic and 42 diabetic individuals by using real-time RT-PCR as described in “Patients and methods”. The data show that IRAK-1 gene expression in non-diabetic individuals correlated positively/significantly with a TLR2 (r = 0.39, P = 0.007) and c MyD88 (r = 0.36, P = 0.01) but did not correlate with b TLR4 (r = −0.04, P = 0.76). IRAK-1 gene expression in diabetic patients did not associate with d TLR2 (r = 0.19, P = 0.20) and e TLR4 (r = −0.06, P = 0.60); however, it correlated significantly with f MyD88 expression (r = 0.52, P = 0.0003). The IRAK-1 correlation with TLR2 and TLR4 is shown for 45 non-diabetic individuals, each, as the missing TLR2/4 data necessitated exclusion of the relevant individuals from correlation analysis