mRNA (A) and GLUT4 protein (B) expression in subcutaneous white adipose tissue. Mice were separated into several groups: lean untreated (CTL, white bars), obese untreated (OB, black bars) or obese atorvastatin-treated (OBA, gray bars) mice. In (A) and (B), relative values of mRNA or protein content (bottom) are shown. In B (top), image of a typical experiment. Slc2a4 mRNA expression was analyzed by Real-time PCR, normalized by Rplp0
(A), and GLUT4 protein expression by Western blotting (B), normalized by total protein analysis with Coomassie Blue-stained gel (C). Data are expressed as means ± S.E. of 5–6 (Slc2a4 mRNA) and 5–6 (GLUT4 protein) animals per group. *P < 0.05 and **P < 0.01 vs. CTL; # P < 0.05 and ## P < 0.01 vs. OB; ANOVA and Student–Newman–Keuls post hoc test.