Figure 1From: Reduced Slc2a4/GLUT4 expression in subcutaneous adipose tissue of monosodium glutamate obese mice is recovered after atorvastatin treatment Slc2a4 mRNA (A) and GLUT4 protein (B) expression in subcutaneous white adipose tissue. Mice were separated into several groups: lean untreated (CTL, white bars), obese untreated (OB, black bars) or obese atorvastatin-treated (OBA, gray bars) mice. In (A) and (B), relative values of mRNA or protein content (bottom) are shown. In B (top), image of a typical experiment. Slc2a4 mRNA expression was analyzed by Real-time PCR, normalized by Rplp0 (A), and GLUT4 protein expression by Western blotting (B), normalized by total protein analysis with Coomassie Blue-stained gel (C). Data are expressed as means ± S.E. of 5–6 (Slc2a4 mRNA) and 5–6 (GLUT4 protein) animals per group. *P < 0.05 and **P < 0.01 vs. CTL; # P < 0.05 and ## P < 0.01 vs. OB; ANOVA and Student–Newman–Keuls post hoc test.Back to article page