Figure 6

Corticosterone-exposed mice display increased alpha-cell mass. Male C57Bl/6J mice were treated with 1% ethanol (white bars) or 100 μg/ml corticosterone (GC, black bars) via the drinking water together with once-daily injections of either PBS (unstriped bars) or liraglutide (Lira) at a final dose of 0.3 mg per kg body weight (striped bars) for five consecutive weeks (n = 5-6). After treatment, the pancreatic gland was dissected and processed for histology. Tissue sections were immunostained for glucagon-positive cells (A). Morphometric analysis was used to determine relative alpha-cell mass (B) and absolute alpha-cell mass either expressed in mg (C) or as μg per g body weight (D). Images in (A) are representative pictures of glucagon staining in the examined tissue (100 × magnification, bar represents 200 μm). Data is shown as mean ± s.e.m. A * denotes a significant (p < 0.05) effect of corticosterone treatment.