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Figure 6 | Diabetology & Metabolic Syndrome

Figure 6

From: Pioglitazone modulates the proliferation and apoptosis of vascular smooth muscle cells via peroxisome proliferators-activated receptor-gamma

Figure 6

Pioglitazone down-regulated the expression levels of the cyclin B1 and cyclin D1 in VSMCs. A, B, The protein levels of cyclin B1 and cyclin D1 from VSMCs treated with GW9662, Pioglitazone, PIO + wt-PPARγ, or PIO + siRNA-PPARγ were analyzed by Western blot, respectively. C, D. The protein levels of cyclin B1 and cyclin D1 from VSMCs transduced with control, Ad-wt-PPARγ or Ad-siRNA-PPARγ were shown. One representative blot is shown. Each experiment has been repeated at least three times. E and F, the mRNA levels of cyclin B1 and cyclin D1 in VSMCs treated with GW9662, Pioglitazone, PIO + wt-PPARγ, or PIO + siRNA-PPARγ were analyzed by real-time RT-PCR. The transcripts of cyclin B1 and cyclin D1 were also determined in PPARγ-overexpressed or -silenced VSMCs. Values are presented as the mean ± SEM. *P < 0.01, **P < 0.001. Each experiment has been repeated three times.

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