Validation of overexpression and silencing of PPARγ. A. Overexpression and silencing of PPARγ in VSMCs. VSMCs were transduced with Ad-mu-PPARγ, Ad-wt-PPARγ or Ad-siRNA-PPARγ, respectively. Cell pellets collected from different groups as indicated were probed against PPARγ and β-actin by Western blot. B. Quantification of PPARγ. The levels of PPARγ from distinct groups in (A) were quantified and normalized to the level of control. *P < 0.05, **P < 0.001. Each experiment has been repeated three times.