Figure 4

Immunofluorescence of retina (A, B, C), iris (D, E, F) and cornea (G, H, I) from a LT-DBT animal (rows: A, D and G) and 57-week-old diabetic (rows: B, E and H) and a LT-Control animal (rows C, F and I). Immunofluorescence of transverse retinal section for Von Willebrand antibody (vWF) (column 1 green) and VEGF (column 2 red) and merge picture (column 3 yellow). In picture A1 the arrow points to the expression of vWF in fiber layer (FL) and arrow head in outer plexiform layer (OPL); in picture A2 the arrow indicates an expression of VEGF in the OPL; in A3 there is a merge picture with arrow in the OPL that indicates the co-expression of the two antibodies (vWF and VEGF). In pictures D1, and D2 the arrows point to the expression of vWF and VEGF in the iris. In pictures G1, G2 and G3 the arrow indicates an abnormal expression of vWF and VEGF in the corneal stroma. 57-week-old animals. Immunofluorescent staining of transverse retinal section (B1, B2 and B3), iris (E1, E2 and E3) and cornea (H1, H2 and H3) for vWF (green) and VEGF (red) and merge. At this time we found no abnormal expression of VW and VEGF. LT-Control animals. Immunofluorescent staining of transverse retinal section in picture C1, C2 and C3: iris in F1, F2 and F3; and cornea in I1, I2 and I3 for vWF antibody (green) and VEGF (red) and merge. No expression was found in control animals. Bar 20 μm.