Figure 1

Immunolocalization of the SGLT1 protein in ductal cells of salivary glands. A. SGLT1 protein in ductal cells of submandibular glands of Wistar Kyoto rats (WKY), diabetic WKY (WKY-D), spontaneously hypertensive rats (SHR) and diabetic SHR (SHR-D). A to D: SGLT1 (green), F-actin (red) and nuclear marker (blue). E to H: only SGLT1 in green color. The SGLT1 protein in ductal cells of WKY can be seen in a very low intensity (A and E), whereas the SGLT1 immunoreactivity is clearly observed in WKY-D (B and F) and SHR (C and G); a further increase in SGLT1 can be observed in SHR-D (D and H). Arrowheads and arrows indicate the absence or presence of the SGLT1 protein in the luminal membrane of ductal cells; respectively. Scale bar, 20 μm. Images are representative of 4 animals in each group. B. SGLT1 protein in ductal cells of the parotid glands from Wistar-Kyoto rats that received physiological saline (A, B, E and F) or propranolol (C, D, G and H), and were subjected to 30-min sympathetic stimulation (B, F, D and H) or not (A, E, C and G). A to D: SGLT1 (green), F-actin (red) and nuclear marker (blue); E to H: only SGLT1 in green color. Scale bar, 20 μm. Arrowheads and arrows indicate the absence or presence of the SGLT1 protein in the luminal membrane of ductal cells; respectively. Images are representative of 4 animals in each group.