Figure 2

CGRP expression in distinct brain regions were compared by measuring the OD of positive cells in brains of control and diabetic rats with immunohistochemical staining. (A). The representative image of CGRP expression in the cerebral cortex of control rats. (B). The representative image of CGRP expression in the cerebral cortex of diabetic rats. (C). The OD comparison of CGRP expression positive cells in cerebral cortex between control and diabetic rats. CGRP expression in the cerebral cortex of diabetic rats significantly decreases compared with control rats (*P < 0.05). (D). The representative image of CGRP expression in the thalamus of control rats. (E). The representative image of CGRP expression in the thalamus of diabetic rats. (F). The OD comparison of CGRP expression positive cells in thalamus between control and diabetic rats. CGRP expression in the thalamus of diabetic rats significantly decreased compared with control rats (*P < 0.05). (G). Schematic representation indicates brain regions where CGRP expression is significantly altered in diabetic rats and the rank order (from more to fewer) of expression quantity change in distinct regions of the integral brain. (H). The comparison of CGRP expression quantity change in distinct brain regions. CGRP expression quantity change is significantly different in distinct brain regions of diabetic rats (*P < 0.05). Bar = 100 μm.