Pleiotropic effects of rosuvastatin on the liver ultrastructure. Mice were fed the standard chow (SC) or a high-fat diet (HF) for 15 weeks, followed by five weeks of rosuvastatin treatment at doses of 10 mg/kg/day (HF-R10), 20 mg/kg/day (HF-R20), or 40 mg/kg/day (HF-R40). Numerical density of mitochondria assessed by stereology. Electron micrographs of the liver (bar = 2 μm). The nucleus (N), nucleolus (Nu) and rugous endoplasmic reticulum (RER) are well preserved, and a number of mitochondria (M) are observed in the SC group. The HF mice exhibit severe macrovesicular (star) and microvesicular (arrow) steatosis, unorganized RER and few mitochondria. Microvesicular steatosis is also found in the HF-R10 group. In contrast, the HF-R20 group has a regular cytoarchitecture, a typical number of mitochondria and spread lipid droplets. A regular nucleus, nucleolus and mitochondria, as well as diminished steatosis, are observed in the HF-R40 group. The symbols indicate a difference compared with [a] the SC group, [b] the HF group and [c] the HF-R10 group.