Figure 4

Expression of apoptosis marker cleaved caspase-3. The cleaved caspase-3 expression is slightly detectable by Western blot and immunofluorescence staining of visceral and subcutaneous fat of WOKW and LEW.1 W rats (Figure 4A, B). Bar represents 15 μm. The extract of Jurkat cells with cytochrome c-induced apoptosis was used in Western blot as the positive control for occurrence of cleaved caspase-3.