Table 1 Sequences for PCR primers of indicated genes of interest
From: AICAR inhibits ceramide biosynthesis in skeletal muscle
| Gene | Sequences |
|---|---|
| 5’-TGGCATTGTTACCAACTGGG | |
| β-actin | 5’-GGGTCATCTTTTCACGGTTG |
| 5’-TACTCAGAGACCTCCAGCTG | |
| SPT1 | 5’-CACCAGGGATATGCTGTCATC |
| 5’-GGAGATGCTGAAGCGGAAC | |
| SPT2 | 5’-GTATGAGCTGCTGACAGGCA |
| 5’-CTGTTCTACTTGGCCTGTTG | |
| CerS1/Lass1 | 5’-TCATGCAGGAAGAACACGAG |
| 5’-TCTTCTCAAAAAGTTCCGAG | |
| CerS2/Lass2 | 5’-AGTGATGATGAAAACGAATGG |
| 5’-TGGCTGCTATTAGTCTGATG | |
| CerS3/Lass3 | 5’-TCAGGATAAAGTAACCCCAG |
| 5’-TGTCGTTCAGCTTGAGTGAG | |
| CerS4/Lass4 | 5’-AGCAGGCTTCACAGAATTTC |
| 5’-CTCCAACGCTCACGAAATTC | |
| CerS5/Lass5 | 5’-ATGCAGACAGAAGATGAGTG |
| 5’-GTTCGGAGCATTCAACGCTG | |
| CerS6/Lass6 | 5’-CTGAGTCGTGAAGACAGAGG |
| 5'-CACCGGTACCTCGGAGCGGA | |
| Des1 | 5'-GTTTGGGATTGATGAACAGGGGT |
| Glucosylceramide | 5’-GCTCAGTACATTGCTGAAGA |
| Synthase | 5’-AGTACGAACCAGAGTTTTGC |
