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Table 1 Sequences for PCR primers of indicated genes of interest

From: AICAR inhibits ceramide biosynthesis in skeletal muscle

Gene Sequences
  5’-TGGCATTGTTACCAACTGGG
β-actin 5’-GGGTCATCTTTTCACGGTTG
  5’-TACTCAGAGACCTCCAGCTG
SPT1 5’-CACCAGGGATATGCTGTCATC
  5’-GGAGATGCTGAAGCGGAAC
SPT2 5’-GTATGAGCTGCTGACAGGCA
  5’-CTGTTCTACTTGGCCTGTTG
CerS1/Lass1 5’-TCATGCAGGAAGAACACGAG
  5’-TCTTCTCAAAAAGTTCCGAG
CerS2/Lass2 5’-AGTGATGATGAAAACGAATGG
  5’-TGGCTGCTATTAGTCTGATG
CerS3/Lass3 5’-TCAGGATAAAGTAACCCCAG
  5’-TGTCGTTCAGCTTGAGTGAG
CerS4/Lass4 5’-AGCAGGCTTCACAGAATTTC
  5’-CTCCAACGCTCACGAAATTC
CerS5/Lass5 5’-ATGCAGACAGAAGATGAGTG
  5’-GTTCGGAGCATTCAACGCTG
CerS6/Lass6 5’-CTGAGTCGTGAAGACAGAGG
  5'-CACCGGTACCTCGGAGCGGA
Des1 5'-GTTTGGGATTGATGAACAGGGGT
Glucosylceramide 5’-GCTCAGTACATTGCTGAAGA
Synthase 5’-AGTACGAACCAGAGTTTTGC