AICAR inhibits SPT2 induction. Male Wistar rats were fed a high-fat diet (HFD) and received daily injections of vehicle (saline) or AICAR (AIC; 0.5 mg/g body weight) for 6 wk. Daily AICAR injections prevented the high-fat diet-induced increase in SPT2 transcription (A) and resulted in decreased SPT2 protein levels (B). *p<0.05 for HFD vs Chow. +p<0.05 for HFD+AICAR vs HFD (n=9-11).