Figure 6
From: Fibronectin glycation increases IGF-I induced proliferation of human aortic smooth muscle cells
Western blot analysis of IGFBP-4 in conditioned media of SMC grown on fibronectin (FN) or AGE-modified FN (AGE-FN). Sub-confluent cells were pre-incubated in serum-free media for 24-h prior to a 24-h exposure to serum-free media (Control) or to 20 ng/mL PDGF-BB (PDGF-BB). A blot representative of three independent experiments is shown. O.D., optical density.