Volume 7 Supplement 1

20th Brazilian Diabetes Society Congress

Open Access

Hba1c levels are increased in patients with gestational diabetes carrying the T/T genotype of the rs1990760 polymorphism in the IFIH1 gene

  • Ana Paula Bouças Kochenborger1Email author,
  • Ana Paula Kutscher Ripoll1,
  • Bianca Marmontel de Souza1,
  • Pamela Sachs Nique1,
  • Denise Alves Sortica1,
  • Natali Cardoso1,
  • Sandra Pinho Silveiro1,
  • Leticia Schwerz Weinert1,
  • Sergio Hofmeister de Almeida Martins Costa1,
  • Luís Henrique Canani1 and
  • Daisy Crispim1
Diabetology & Metabolic Syndrome20157(Suppl 1):A75

DOI: 10.1186/1758-5996-7-S1-A75

Published: 11 November 2015

Background

Gestational diabetes mellitus (GDM) is diabetes diagnosed in the second or third trimester of pregnancy that is not clearly overt diabetes. This condition is a common complication of pregnancy, being associated with both maternal and neonatal adverse outcomes. Several studies have indicated that viruses play an important role in the pathophysiology of diabetes. IFIH1/MDA5 gene encodes a cytoplasmic receptor that recognizes viral nucleic acids, playing a major role in the immune response against viruses. Accordingly, the rs1990760 (G/A) polymorphism in the IFIH1 gene has been associated with type 1 diabetes mellitus in different populations. Considering that the MDA5 receptor is expressed in human placenta, we therefore hypothesized that the rs1990760 G/A polymorphism could be also associated with GDM.

Objectives

To investigate the association between the IFIH1 rs1990760 polymorphism and GDM and/or its clinical characteristics in a Southern Brazilian population. Moreover, we aimed to analyze IFIH1 expression in placentas from GDM patients and healthy pregnant women according to different rs1990760 genotypes.

Materials and methods

We analyzed 129 patients with GDM (cases) and 144 pregnant women without GDM (controls). The polymorphism was genotyped by RT-PCR using TaqMan MGB probes (Life Technologies). IFIH1 expressions in placentas from 70 cases and 36 controls were evaluated using RT-qPCR.

Results

Genotype and allele frequencies of the rs1990760 polymorphism did not differ between GDM patients and nondiabetic controls (P=0.702 and P=0.708, respectively), and adjustment for ethnicity did not change these Results. In GDM patients, fasting glucose levels, body mass index and age were not significantly different between rs1990760 genotypes. However, T/T genotype carriers had increased levels of glycated hemoglobin (A1c) as compared to G allele carriers (5.9 ± 0.4 vs. 5.4 ± 0.5, P=0.007). IFIH1 expression in placenta was similar among the three genotypes of the rs1990760 polymorphism (P>0.05). Interestingly, IFIH1 expression in placenta was decreased in patients with GDM as compared to controls (7.0 ± 4.2 vs. 9.7 ± 9.4, P=0.044). Accordingly, IFIH1 expression was inversely correlated to A1c levels (r=0.549, P=0.035).

Conclusions

This study suggests an association between the T/T genotype with increased levels of A1c. Furthermore, IFIH1 gene expression seems to be associated with protection for GDM, and it was inversely correlated to A1c levels.

Authors’ Affiliations

(1)
HCPA

Copyright

© Kochenborger et al. 2015

This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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